Journal: Cell metabolism
Article Title: Translational and HIF-1α-dependent metabolic reprogramming underpin metabolic plasticity and determine responses to kinase inhibitors and biguanides.
doi: 10.1016/j.cmet.2018.09.001
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Recombinant DNA pLKO.1 Non-Target shRNA Control Sigma-Aldrich SHC002 pLKO.1 hHIF-1α shRNA Sigma-Aldrich TRCN0000003810 pLKO.1 hIDH1 shRNA Sigma-Aldrich TRCN0000027298 a pLKO.1-neo vector control a gift from Sheila Stewart Addgene 13425 pLKO.1-neo-mIDH1 shRNA This paper N/A pLV-Hygro-U6 mPHGDH shRNA This paper N/A pLV-Hygro-U6 mASNS shRNA This paper N/A hCas9 ( Mali et al., 2013 ) Addgene 41815 CRISPR gRNA h4E-BP1 GeneCopoeia HCP204676-SG01–3-B-a CRISPR gRNA h4E-BP1 GeneCopoeia HCP204676-SG01–3-B-b CRISPR gRNA h4E-BP1 GeneCopoeia HCP204676-SG01–3-B-c CRISPR gRNA h4E-BP2 GeneCopoeia HCP254214-SG01–3-B-a CRISPR gRNA h4E-BP2 GeneCopoeia HCP254214-SG01–3-B-b CRISPR gRNA h4E-BP2 GeneCopoeia HCP254214-SG01–3-B-c CRISPR gRNA m4E-BP1 GeneCopoeia MCP227000-SG01–3-B-a CRISPR gRNA m4E-BP1 GeneCopoeia MCP227000-SG01–3-B-b CRISPR gRNA m4E-BP1 GeneCopoeia MCP227000-SG01–3-B-c CRISPR gRNA m4E-BP2 GeneCopoeia MCP229088-SG01–3-B-a CRISPR gRNA m4E-BP2 GeneCopoeia MCP229088-SG01–3-B-b CRISPR gRNA m4E-BP2 GeneCopoeia MCP229088-SG01–3-B-c pBabe-puro ( Dowling et al., 2010 ) N/A pQCXIB (blast) ( Campeau et al., 2009 ) Addgene 17487 pLV-Neo-CMV-4E-BP1 This study N/A Software and Algorithms R The R Project for Statistical Computing N/A ImageJ NCBI https://imagej.nih.gov/ij/index.html Chemstation Agilent N/A MassHunter Agilent N/A Aperio Imagescope Leica Biosystems N/A FlowJo FlowJo, LLC N/A FacsDiva BD Biosciences N/A GraphPad Prism 7.0 GraphPad Software https://www.graphpad.com/ Deposited Data Raw and analyzed data This paper https://data.mendeley.com/datasets/4k6knxb996 Open in a separate window KEY RESOURCES TABLE
Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, In Situ, Detection Assay, shRNA, Control, Plasmid Preparation, CRISPR, Software
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